My Curious thoughts

  From the early childhood (and till now) I have always faced problems in swallowing encapsulated drugs. I have always avoided these kinda capsulated pills and always had the doctor to refer me tablets or liquid alternatives instead. I got to know that this kind of difficulty in swallowing is called dysphagia. Though it is medically not recommended to cut open or chew a capsule pill, but not gonna lie I have done this few times myself. Here’s a link that shares tips to swallow a pill which may be helpful to those who are facing a similar problem like me: https://www.healthline.com/health/how-to-swallow-a-pill#help-a-child So stemming from my inability to ingest big pills and capsules I wondered why some of these drugs had to be necessarily inside this hard-to-swallow-capsule? Well some of the drugs had necessarily to be in liquid form trapped inside the solid capsule (often made up of gelatin ; which is inert and easily degraded in the body without any toxic side effects). Liquid form

 I have seen many people (including me) struggle to find a good practical protocol with principle, roles of reagents , observations and discussions all clubbed together as one. The literature is always dispersed throughout net across various papers and documentation. Therefore here I'm providing a summarized  short but to the point practical for the Phenol-Chloroform Method of genomic DNA isolation from Bacteria(say E.coli). ------------------------------------------------------------------------------------------------------------------------------   AIM Isolation of genomic DNA from bacterial cells MATERIALS REQUIRED LB medium ( per ml, 10 mg tryptone, 5 mg yeast extract, and 5 or 10 mg NaCl), Incubator   Reagents  20% SDS , Phenol: chloroform: isoamylalcohol (25:24:1) , RNase , NaCl solution (5M) , chilled ethanol(70%) , TE(Tris-EDTA) buffer[10mM Tris-Cl(pH8.0), 1mM EDTA(pH 8.0] , Lysis buffer[TE buffer, proteinase K, NaCl/SDS]